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This experiment was conducted to investigate the effects of SCFA& MCFA and Lactobacillus on the growth and immune performance of macrobrachium nipponense.
In this study, 6 treatment groups were set up, G0 group was the blank control group, G1~G5 groups were Lactobacillus group, 0.15% GML group, 0.15% glyceryl butyrate group, and 0.15% GML group + 0.15% glyceryl butyrate group, 0.2% GML + 0.2% glyceryl butyrate group. All treatment groups (G1~G5 groups) need to be mixed with Lactobacillus bacteria solution by spraying at a ratio of 1:4 (bacteria solution/mL:feed/g) when feeding.
In this study, the growth indicators of macrobrachium nipponense, the expression of various immune response factors and the activity of immune-related biological enzymes were measured.
The results show:
1. Compared with the control group, the growth and weight gain rates of the G1 and G4 groups were increased by 24.99% and 26.69% respectively (P<0.05), and the specific growth rates of the test groups G1, G4 and G5 were respectively increased by 18.92%, 18.92% and 14.41% compared with the control group (P <0.05).
2. In terms of serum immunity, the content of malondialdehyde (MDA) in G1 and G3 groups increased by 60.71% and 47.61% respectively compared with the control group (P<0.05); the content of catalase (CAT) in G2, G4, G5 Compared with the control group, the control group increased by 219.22%, 140.94%, and 431.68% respectively (P<0.05).
3. In terms of total superoxide dismutase (T-SOD) activity, the G2, G3, and G5 groups increased by 50.92%, 62.77%, and 115.36%, respectively, compared with the control group. The immune-related glutathione peroxidase (GSH-Px) activity in the hepatopancreas was increased by 50.37%, 56.09%, and 50.66% in the G1, G3, and G5 groups compared with the control group, respectively (P<0.05);
4. The expression of immune-related response genes Myd88, HSP60 and HSP70 also increased in different degrees in each test group compared with the control group.
Conclusion:
The combination of a certain concentration of medium and short-chain fatty acid glycerides and Lactobacillus can significantly improve the growth and immune performance of macrobrachium nipponensis.
Effects of Different Additives on Growth Performance of macrobrachium nipponensis.
Effects of Different Additive Treatments on Antioxidative Ability of macrobrachium nipponensis.
